Part:BBa_M12203:Design

Amino Acids 176-240 of E0040

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 119
  

Design Notes

Not applicable. Forward primer: gtttcttcgaattcgcggccgcttctagaggttcaactagcagaccattatcaacaaaatac Forward primer: gtttcttcgaattcgcggccgcttctagagcaactagcagaccattatcaacaaaatactc Reverse primer: gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg Reverse primer: gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg

M12203f1: ttcttcgaattcgcggccgcttctagaggttcaactagcagaccattatcaacaaaatac

M12203f2: tttcttcgaattcgcggccgcttctagagcaactagcagaccattatcaacaaaatactc

M12203r: tttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg

check designs to see what is right

Source

BBa_E0040.